酶标法检测小巨核细胞对单纯性贫血的诊断价值

DETECTION OF MICROMEGAKARYOCYTES BY ENZYME LINKED IMMUNOSORBENTASSAY: ITS VALUE IN DIFFERENTAIL DIAGNOSIS OF SIMPLE ANEMIA

目的探讨酶标法检测小巨核细胞在单纯性贫血诊断和鉴别诊断中的意义。方法应用碱性磷酸酶-抗碱性磷酸酶桥联酶联技术(APAAP)法和瑞特染色法,分别检测106例单纯性贫血(包括骨髓增生异常综合征20例,缺铁性贫血18例,巨幼细胞性贫血10例,溶血性贫血18例,多发性骨髓瘤20例,骨髓纤维化7例,肾性贫血5例,纯红细胞再生障碍性贫血3例,慢性病贫血3例,慢性再生障碍性贫血2例)病人骨髓中巨核细胞的数量和小巨核细胞的数量及类型。结果APAAP法检测到的巨核细胞的数量较瑞特染色法多,APAAP法检测小巨核细胞的阳性率较瑞特染色法高,差异有显著性(χ2=5.013,P<0.05)。结论APAAP法检测小巨核细胞明显优于瑞特染色法,是诊断单纯性贫血类型的重要方法。

Objective To assess the significance of detection of micromegakaryoeytes by enzyme linked immunosorbentassay in differentia; diagnosis of simple anemia. Methods Bone marrow smears from patients with different kinds of anemia （n= 106） were detected by both APAAP staining and Wright＇s staining for the number and types of micromegakaryocytes, Results The number of micromegakaryoeytes and their positive rate detected by APAAP staining were significantly higher than that of Wright＇s method （x^2=5,013, P〈0,05）, Conclusion APAAP staining obviously surpasses Wright＇s method in the detection of micromegakaryocytes, which is an important technique in the diagnosis of simple anemia.