摘要
研究了氧化亚铁钩端螺菌(Leptospirillums ferriphioum)发酵罐补料培养的最佳条件。结果表明:pH为1.2,接种量为33%,充气量为6L/m in,搅拌转速为450 r/m in时,培养30h后,用3m l/m in的速度补料,补料1 500m l后,菌密度可达1.0×1010个/m。
The article studied the optimum condition of the fed-batch technology on Leptospirillums ferriphioum. The result showed that the optimum fermentation condition was as follows： pH was 1.2, inoculation quantity was 33%, air flow was 6L/min, the stirring speed was 450r/min, after 30h culture time adding up the material at the 3ml/min speed and the batch quality was 1 500ml. The bacteria density reach 1.0 ×10^10 per liter.
出处
《黄金》
CAS
北大核心
2006年第9期35-37,共3页
Gold
基金
东华理工学院硕博启动基金(DSH0510)
关键词
氧化亚铁钩端螺菌
高密度
补料法
Leptospirillums ferriphioum
high density
fed-batch fermentation
