小鼠睾丸生殖细胞减数分裂标本制作方法的改进

A MODIFICATION FOR PREPARING MEIOSIS SPECIMEN OF GENERATIVE CELLS OF MOUSE TESTICLE

目的对小鼠睾丸生殖细胞减数分裂标本制作方法加以改进,以便使各期分裂细胞增多、背景清晰、结构紧密、图像清楚、利于阅片。方法小鼠肌肉注射6.5 mg/kg丙酸睾丸酮后继续饲养48 h;处死前腹腔注射秋水仙素,在标本制作过程中采用低渗处理、脱水、中性树胶封片。结果低倍镜下可见细线期、偶线期、粗线期、双线期、终变期细胞,各期细胞染色体分裂相较常规制片明显增加。结论方法改进后可见大量分散良好、背景清晰、染色体结构紧凑的生殖细胞减数分裂各期分裂相。

Objective To improve the meiosis-speclmen-making methods so as to get better specimen with more dividing cells, clear background, compact chromosome structure, and distinct image. Methods The mice were injected intramuscularly with testosterone propionate （6.5 mg/kg） and bred for 48 h. Generative cells were harvested from testicle and treated with colchicines. The generative cells underwent hypotonic treatment and dehydration, and were mounted with neutral gum. Results All dividing stages （leptotene, zygotene, pachytene, diplotene stages and diakinesis） were observed in low power lens and those dividing stages increased greatly compared with the routine method. Conclusion Each dividing stage can be observed with clear background, compact chromosome structure and good dispersion with this modified method.