阻抑动力学荧光法测定吡拉西坦

Inhibitory kinetic fluorimetric determination of piracetam

在酸性介质中，吡拉西坦能够阻抑过硫酸钾氧化荧光桃红的荧光淬灭反应，从而建立了阻抑动力学荧光法测定吡拉西坦含量的新方法，吡拉西坦的质量浓度与荧光强度的变化在0．016-0．112g·mL^-1范围内呈良好线性关系，相对标准偏差（RSD）为1．52％，方法的检出限为为2．8×10^-3g·mL^-1．该法简便、快速，结果可靠，并应用于药物制剂中吡拉西坦含量的测定，结果令人满意．

An inhibitory Kinetic Fluorimetric method is reported for the determination of piracetam , The proposed method is based on the inhibitory effect of piracetam on the reaction of K28208 oxidation of berberine, catalyzed in citric acid/sodium citrate buffer solution medium, The detection limit for ampicillin is 2.8×10^-3 g·mL^-1, The linear range of the determination is 0.016-0.112g· mL^-1. This method has been used successfully to determine piracetam troche.