贲门癌端粒酶活性表达及与p53基因突变关系的研究

A Study the Relationship of Telomerase Activity and p53 Gene Mutation in Cardiac Cancer

目的探讨贲门癌组织中端粒酶活性及与p53基因突变的关系。方法采用TRAP-ELSIA、PCR-SSCP方法对46例贲门癌组织、配对癌旁黏膜及30例手术残端正常黏膜中端粒酶活性、p53基因突变进行研究。结果贲门癌癌灶、配对癌旁及正常黏膜端粒酶活性表达率分别为82.61%(38/46)、43.48%(20/46)、13.33%(4/30)。p53基因Exon5→8的突变率分别为39.13%(18/46)、14.35%(2/46)、0。上述两组数值癌与非癌组相比均有统计学意义(P<0.01)。端粒酶-A值癌组1.89±0.41,癌旁1.49±0.43,正常组0.54±0.45,癌与非癌组比较有统计学意义(P<0.05)。端粒酶活性表达组p53基因突变率44.74%(17/38),未表达端粒酶活性组p53基因突变率12.50%(1/8),组间比较有统计学意义。结论贲门癌端粒酶活性表达率及端粒酶-A值均明显高于非癌组织,表明端粒酶激活与贲门癌的发生密切相关。贲门癌组织中p53基因突变明显高于非癌组织,且端粒酶活性表达组p53基因突变率明显高于非表达组,提示p53基因突变使其抑癌功能丧失并促发端粒酶活性,导致贲门癌的发生。

Objective To study the relationship of the telomerase activity and the p53 gene mutation in cardiac cancer. Methods Telomerase activity and the p53 gene mutation were detected in 46 case with cardiac cancer , peri-cancerous mucosa and 30 cases of normal mucosa by TRAP- ELISA and PCR-SSCP.Results The rates of expression of telomerase activity in cardiac cancer, peri-cancerous and normal mucosa were 82.61% （38/46） ,43.48% （20/46） , 13.33% （4/30） .The rates of Exon 5→8 of p53 gene mutation were 39.13% （ 18/46）, 14.35% （2/46） ,0 there was significant difference between group cancer and without cancer, the expression（ P 〈 0.01 ）. mean of A value of telomerase is 1.89 ± 0.41 in cancer group and were 1.49 ± 0.43,0.54 ± 0.45 respectively in peri-cancerous and normal mucosa, there was significant difference in cancer group and group without cancer（ P 〈 0.05）. the rate of p53 gene mutations in group of expression of telomerase activit was 44.74% （ 17/38）, and was 12.50% （1/8） in without expression of telomerase activit. There was significant difference between the two groups . Conclusions The rates of expression of telomerase activity and mean of A value of telomerase in cardiac cancer were obviously higher than that without cancer . indicated telomerase activity was closely related with the occurrence of cardiac cancer . p53 gene mutations in cardiac cancer, were higher than the tissues of group without cancer, and the rate of p53 gene mutation in telomerase activit was obviously higher than that of group without cancer, this showed that the p53 gene mutation could lose the function of suppressing cancer and prompt telomerase activity and cause the cardiac cancer.