扁穗牛鞭草RAPD影响因素的研究

Study on influencing factors of RAPD in Hemarthria compressa

以扁穗牛鞭草Hemarthria compressa基因组DNA为模板，通过单因子试验分别研究了RAPD反应体系中主要成分：Mg^2＋浓度、dNTP浓度、引物浓度、模板浓度、TaqDNA聚合酶用量以及BSA浓度对RAPD-PCR扩增的影响，找出各自的最适条件，建立了适合扁穗牛鞭草的RAPD反应体系：在25μL反应体系中，内含1×PCRbuffer、1．5～2mmol／L Mg^2＋、150μmol／L dNTP、20ng引物、40ng模板、1 U TaqDNA聚合酶。

Based on the genomic DNA extracted from Hemarthria compressa, the main elements,i, e. Mg^2＋ concentration, dNTP concentration, primer concentration, template DNA concentration, Taq DNA polymerase dosage on RAPD-PCR amplification and BSA concentration were tested by single factor experiment, to determine their optimal levels respectively. A reaction system suitable for H. compressa was established, that is, 25 μL amplification reactions system containing 1 ×PCR buffer,1.5-2 mmol/L Mg^2＋ ,150 μmol/L dNTP,20 ng primer,40 ng template DNA 、1U Taq DNA polymerase.