霍山石斛杂交种与其亲本生长生理指标及药效成分含量的比较

Comparison on growth,physiology and medicinal components of Dendrobium huoshanense hybrid and its parents

目的:对霍山石斛杂交种与其亲本进行生长、生理指标及主要药效成分含量的比较,为霍山石斛种质资源改良提供理论依据。方法:叶绿素含量用无水乙醇法,光合速率用CI-310便携式光合作用测定系统测定;多糖含量用浓硫酸-苯酚比色法,总生物碱含量用溴甲酚绿酸性染料比色法测定。结果:杂交种生长量积累表现为近铜皮石斛的特性,较霍山石斛有极大优势;一年生杂交种叶绿素含量及光合速率均极显著高于其亲本;杂交种二年茎与三年茎多糖含量和总生物碱含量均与霍山石斛接近。结论:杂交种在生长和药效成分积累方面结合了2个亲本之间的优势,具有很大的开发利用前景。

Objective： To compare the hybrid between species of Dendrobium huoshanense and its parents on growing, physiologic indexes and content of medicinal components, and provide theoretical basis for species quality improvement. Method： The chlorophyll content, the photosynthesis rate, the polysaccharides content and the alkaloids content were measured by anhydrous ethanol method, C1-310 photosynthesis determination system, colorimetry of concentrated sulphuric acid-phenol and acid dyes colorimetry respectively. Result： The growth of hybrid was close to D. moniliforme, and apparently higher than D. huoshanense. The chlorophyll content and the photosynthesis rate of one-year-hybrid were markedly higher than its parents. The content of polysaccharides and alkaloids in two-year-stem and three-year-stem of hybrid were close to that of D. huoshanense. Conclusion： The hybrid integrates superiority of parents on growth and accumulation of medicinal components opens vast vistas for development and utilization.