血脂康与辛伐他汀治疗大鼠脑缺血-再灌注损伤的研究

Effects of Xuezhikang and simvastatin on cerebral ischemia-reperfusion injury in rat

目的:比较血脂康与辛伐他汀对大鼠脑缺血-再灌注损伤的作用,并观察L-硝基精氨酸甲酯(L-NAME)干预的影响。方法:大鼠每日用血脂康(2·4g·kg-1)、辛伐他汀(80mg·kg-1)或生理盐水连续灌胃2周,血脂康和辛伐他汀治疗的大鼠各取一半于脑缺血前45min经侧脑室注射L-NAME,后用线栓法制作大鼠脑缺血-再灌注损伤模型;于再灌注后2,24,48h进行神经功能评分;采用TTC染色法测定脑梗死体积并检测脑组织脂质过氧化产物丙二醛含量。结果:血脂康与辛伐他汀均能减少大鼠脑缺血-再灌注损伤后的脑梗死体积和脑组织脂质过氧化产物含量,并改善功能,与溶媒组比较有显著性差异;L-NAME能部分抑制血脂康和完全抑制辛伐他汀的上述保护作用。结论:血脂康对大鼠脑缺血-再灌注损伤具有保护作用,其保护作用部分与其含他汀类成分有关。

Objective： To observe the effects of Xuezhikang and simvastatin on cerebral ischemia/reperfusion injury in rat, as well as the influences after intervention with L-NAME. Method： Rats were given orally with Xuezhikang and simvastatin or vehicle for 2 weeks, and then subjected to middle cerebral artery occlusion for 120 min using intraluminal filament model. L-NAME were injected into the lateral ventricles in half of the rats treated with Xuezhikang and simvastatin 45 min before the ischemia. The neurological deficits examinations were performed at 2, 24, 48 h after reperfusion. After the last examination the animals were sacrificed, the infarct volumes were determined by TTC staining, and MDA levels were also measured. Result： Xuezhikang and simvastatin both significantly reduced the infarct volume and improved the functional recovery when compared to vehicle. Xuezhikang and simvastatin both significantly decreased the MDA accumulation after reperfusion. L-NAME partially inhibited the protective effect of Xuezhikang but nearly completely abolished the protective effect of simvastatin. Conclusion： Xuezhikang has protective effects on ischemic brain damage in rats, which the beneficial effects are partly due to the statins components. The other components in Xuezhikang may also account for the neuroprotective effects, which is worth further investigations.