黄连解毒汤抗肿瘤作用的实验研究

Study on antitumor activities of Huanglian Jiedu decoction

目的:研究黄连解毒汤的抗肿瘤作用。方法:采用H22小鼠肝癌实体瘤移植性肿瘤模型进行体内实验,通过检测抑瘤率、胸腺指数、脾脏指数等指标观察黄连解毒汤的体内抑瘤作用及对免疫器官的影响。并运用血清药理学方法研究了对人结肠癌Swille,人肺腺癌SPC-A-1,人胃癌SGC-7901,人乳腺癌细胞MCF-7体外生长的抑制作用。结果:对荷瘤小鼠肝癌H22在体内有抗肿瘤活性,抑瘤率大、中、小剂量组分别为45·45%(P<0·001),28·73%(P<0·05),17·78%。含药血清在体外用MTT法测试结果显示,对4种人瘤株,大、中剂量组抑瘤作用均呈现极显著差异(P<0·001)。结论:黄连解毒汤对H22小鼠肝癌实体瘤有一定的体内抗肿瘤活性,并具剂量依赖性;其含药血清体外对4种人的肿瘤细胞也有一定抑制作用。

Objective： To study the antitumor activity of Huanglian Jiedu decoction （HLJDT）. Method： Antitumor activities were tested in mice with experimental tumor H22 in vivo, and the thymus index, spleen index and tumor inhibitory rate were evaluated. The effects on cancer cells from human were investigated in vitro using serum pharmacological approach. Swille, SPC-A-1, SGC-7901 and MCF-7 cancer cells were incubated in culture media containing serum from mice medicated with HLJDT. The inhibitory effects of HLJDT serum were observed by MTT assay. Result： HLJDT showed significant antitumor activities on H22 in mice. All of the HIJDT serum in different dosage groups could highly inhibit the proliferation of 4 cancer cell lines from human. Conclnsion： The HIJDT can significantly inhibit the tumor H22 in mice in a dose-dependent manner, the drug serum has obvious anticancer effects against Swille, SPC-A-1, SGC-7901 and MCF-7.