历节胶囊对AA大鼠淋巴细胞中Fas和FasL基因表达的影响

Influences of Lijie Capsules on expressions of Fas and FasL genes in lymphocytes in rats with adjuvant arthritis

目的研究历节胶囊对佐剂型关节炎大鼠外周血淋巴细胞中Fas和FasL基因表达的影响。方法以佐剂型关节炎大鼠作为实验研究对象,通过实时荧光定量PCR的方法,半定量检测该药对外周血淋巴细胞Fas和FasL基因表达的影响。结果治疗组Fas基因表达均有不同程度的提高,但与模型组相比表达量无显著性差异；历节胶囊治疗组可以使FasL基因表达显著提高(P＜0．05)。结论历节胶囊可以通过调节Fas/FasL介导的凋亡系统,诱导细胞毒性T细胞的凋亡发生,维持机体的免疫稳态,缓解因自身免疫所导致的关节炎症反应,使疾病得以向愈。这可能是历节胶囊治疗类风湿性关节炎的作用机制之一。

Objective To study the influences of Lijie Capsules on the expressions of Fas and FasL genes in lymphocytes of peripheral blood in rats with adjuvant arthritis （ AA ）. Method To detect semiquantitatively the influence of Lijie Capsules on the expressions of Fas and FasL genes in lymphocytes of peripheral blood in AA rats by polymerase chain reaction （PCR） of real-time fluorescent. Result In the treatment group the Fas gene expression increased but there was no significant difference compared with that of the model group, while FasL gene expression increased obviously （ P 〈 0. 05 ） in Lijie Capsules group. Conclusion Lijie Capsules could induce the apoptosis of toxic T cells by regulating the apoptotic system mediated by Fas/FasL genes, keep the balance of body immunity, relieve the arthritis reaction induced by autoimmunity and improve the disease recovery. It may be one of the mechanisms of Lijie Capsules administrated in the treatment of rheumatoid arthritis.