鹅膏蕈的氧化损伤及白藜芦醇的抗损伤作用观察

Oxidative damage induced by phalloides and protective effect of white pigweed mellow

目的:探讨鹅膏蕈的氧化损伤及白藜芦醇的抗损伤作用。方法:将4组SD大鼠分别给予不同剂量的鹅膏覃毒素(腹腔注射),其中2组给予不同剂量的白藜芦醇(腹腔注射)。观察各组染毒后不同阶段的血液生化酶、脏器系数和氧化损伤指标。结果:与空白对照组比较,鹅膏蕈高剂量组超氧化物歧化酶(SOD)活力与丙二醛(MDA)含量变化有显著差异(P<0.05),动物肝脏脏器系数明显增大变化有显著差异(P<0.05),肝脏功能指标丙氨酸转氨酶(ALT)、心肌损伤特异性标志物天冬氨酸转氨酶(AST)活力水平变化有显著差异(P<0.05)。给予白藜芦醇可减轻指标改变的程度。结论:鹅膏蕈毒素可引起氧化损伤作用,白藜芦醇可减轻这种损害程度。

Objective： To explore the oxidative damage induced by phalloides and the protective effect of white pigweed mellow on the damage. Methods： Fifty SD rats were randomly divided into 5 groups （ n = 10 in each group） ,including a control group and 4 experiment groups. The rats from each experiment group was intraperitoneally injected with various dosages of phalloides,and those from 2 of 4 experiment groups were also intraperitoneally injected with white pigweed mellow. The changes of biochemical enzymes, organ index, and index of oxidative damage were measured at different intervals after poisoning. Results： There were significant differences in the acitivity of superoxide dismutase （ SOD）, the content of malondialdehyde （MDA） , the organ index, and the activities of alanine aminotransferase and aspartate aminotransferase between the control group and the group treated with high-dose phalloides （ all P 〈 0. 05）. The injection of white pigweed mellow relieved the above changes. Conclusion ： Phalloides can induce oxidative damage, which can be reduced by white pigweed mellow.