参附注射液对肝缺血再灌注大鼠微循环的影响

Effect of Shenfu injection on the microcirculation during hepatic ischemia and reperfusion in rats

目的:应用大鼠肝缺血再灌注(IR)损伤模型探讨参附注射液对肝微循环的影响。方法:12只W istar大鼠随机分为IR组和SF组。SF组给予参附注射液10 m l/kg。IR组大鼠给予相同剂量的生理盐水。两组均采用Pringle法阻断肝门缺血15m in再灌注3 h,测定血清生化酶ALT、AST、LDH和血浆TXB2、6-keto-PGF1α,及观察肝组织形态学改变。结果:SF组血清ALT、AST、LDH水平低于IR组;血浆TXB2低于IR组,6-keto-PGF1α高于IR组,二者比值TXB2/6-keto-PGF1α低于IR组;SF组肝实质细胞和肝窦内皮细胞损伤明显减轻,肝窦形态的改变和肝窦内的瘀血、白细胞聚集也明显减轻。结论:参附注射液通过保护肝窦内皮细胞,降低TXA2/PG I2比值及减轻肝窦内炎性反应,改善肝脏微循环灌注。

Objective： To explore the effect of Shenfu injection on the microcirculation during hepatic ischemia and reperfusion in rats. Methods： Twelve male Wistar rats weighing 200 to 250 g were randomly divided into 2 groups ： SF group （ rats were intraperitoneally injected with Shenfu injection of 10 ml/kg） and IR group （ rats were injected with 0.9% sodium chloride solution at the same dose of Shenfu injection）. Hepatic ischemia was caused by Pringle＇ s maneuver and lasted for 15 minutes, and then 3-hour reperfusion was performed. Arterial blood samples for liver function tests and venous blood samples for the measurement of thromboxane B2 （ TXB2 ） and 6-keto-PGF1α were collected 3 hours after reperfusion. Liver tissues were sampled 3 hours after reperfusion and fixed in 2.5% glutaraldehyde solution and 10% formalin solution respectively for morphological study. Results： Compared with IR group, in SF group, the levels of liver enzymes （ alanine aminotransferase, aspirate aminotransferase, and lactate dehydrogenase） in serum, the level of TXB2 in plasma, and the ratio of TXB2 to 6-keto-PGF1α were lower, while the level of 6-keto-PGF1α in plasma was higher. Histopathological examination showed that 3-hour reperfusion after 15-minute ischemia caused severe hepatic histological alterations. Marked structural abnormalities were observed in IR group. In SF group, hepatic tissue injury was significantly reduced. Conclusion： Shenfu injection protects hepatic tissues from ischemia-reperfusion injury by defending sinusoidal endothelial cell, alleviating leukocyte aggregation, and reducing the ratio of thromboxane A2 to prostacyclin, and thus improves hepatic microvascular perfusion.