N-乙酰半胱氨酸和α-硫辛酸对汞氧化损伤的拮抗作用

Protective effect of N-acetyl-L-cysteine and α-lipoic acid on the oxidative injury induced by mercury

目的:探讨N-乙酰半胱氨酸(NAC)和α-硫辛酸(LA)对汞急性毒性的影响。方法:W istar大鼠32只,随机分成4组:对照组、单纯染汞组、NAC干预组、LA干预组。注射48 h后,测定血清谷丙转氨酶(GPT)活性,肾皮质、肝脏中汞、丙二醛(MDA)和谷胱甘肽(GSH)含量及谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性。结果:与对照组比较,单纯染汞组肾皮质、肝脏汞含量增高,血清GPT活性升高;肾皮质、肝脏MDA含量显著升高,GSH含量和GSH-Px、SOD活性降低。与单纯染汞组比较,NAC干预组血清GPT活性,肾皮质、肝脏MDA含量显著降低;GSH含量、GSH-Px活性显著增高;肝脏SOD活性增高。LA干预组肾皮质汞含量升高,肝脏汞含量、血清GPT活性显著降低;肾皮质、肝脏MDA含量降低,GSH含量和GSH-Px活性增高;肝脏SOD活性增高。结论:NAC和LA预处理对汞所致急性肝肾氧化损伤有一定的保护作用。

Objective： To study the effect of N-acetyl-L-cysteine （NAC） and α-lipoic acid （LA） on the acute toxicity of mercury. Methods： Thirty-two Wistar rats were randomly divided into 4 groups （n = 8） ： control group, mercury group, NAC pretreatment group, and LA pretreatment group. The rats were killed 48 hours after the last injection, and the activity of glutamic-pyruvic transaminase （GPT） in serum and the contents of mercury, malondialdehyde （MDA） , and glutathione （GSH） and the activities of glutathioneperoxidase （GSH-Px） and superoxide dismutase （SOD） in the liver and renal cortex were determined. Results： Compared with the control group, in mercury group, the activity of GPT in serum and the contents of mercury and MDA in the liver and renal cortex significantly increased; the content of GSH and the activities of GSH-Px and SOD in the liver and renal cortex significantly decreased. Compared with mercury group, in NAC and LA pretreatment groups, the activity of GPT in serum and the content of MDA in liver and renal cortex significantly decreased ; the content of GSH and the activity of GSH-Px in liver and renal cortex and the activity of SOD in liver significant increased. Compared with mercury group, in LA pretreatment group, the content of mercury significantly decreased in liver and significantly increased in renal cortex. Conclusion：Pretreatment with NAC or LA could protect against the acute liver and kidney oxidative injuries induced by mercury.