喹唑啉分子印迹聚合物微球的吸附特性

Study of Binding Characteristics of Quinazoline Molecularly Imprinted Polymeric Microspheres

以2,4-二氯-6,7-二甲氧基喹唑啉(DCQAL)为模板分子,采用单步溶胀聚合法制备了粒径均匀的分子印迹聚合物微球(M IPM s),并用静态吸附法研究了功能单体、三乙胺及水对M IPM s吸附性能的影响,考察了微球的识别性能。制得的M IPM s主要靠氢键作用吸附与识别模板分子,甲基丙烯酸(MAA)与模板分子间能产生较强的氢键作用,以其为功能单体制得的M IPM s具有较好的吸附与识别性能,其高亲和力与低亲和力结合点的最大表观吸附量分别为17.53μmol/g和117.02μmol/g,以4-氯-6,7-二甲氧基喹唑啉(CQAL)为竞争底物,其分离因子α达1.78。往吸附液中添加三乙胺或少量水会减弱M IPM s的吸附能力。

Uniformly sized molecularly imprinted polymeric microspheres （MIPMs） were prepared by single-step swelling polymerization using 2,4-dichloro-6,7-dimethoxyquinazoline （DCQAL） as template molecules. Static adsorption was employed to study the effecis of the functional monomers, triethylamine and water on binding characteristics of the MIPMs for DCQAL, and the recognition properties were also investigated by the same way. MIPMs adsorbed and recognised template molecule mainly by hydrogen bond action. The recognition and binding properties of the MIPMs prepared by methacrylic acid were good due to its strong hydrogen bond with the template, the apparent maximum numbers of binding sites with high affinity and low affinity being 17.53 μmol/g and 117.02 μmol/g respectively. The separation factor was up to 1.78 when 4-chloro-6,7-dimethoxyquinazoline（CQAL） was chosen as the competitive molecule. Using triethylamine or a small quantity of water as additive in adsorption solution could obviously decreased the binding properties of MIPMs.