亚热带植物叶蛋白酶活性研究

Determination of Leaf Protease Activity of Subtropical Plant

为探索新的植物蛋白酶，采用改进的Kunit蛋白酶活性测定法，对分属61科的190种亚热带植物叶中的蛋白酶活性进行了分析。结果表明，叶蛋白酶活性低于1000U／g的植物有133种，介于1000-2000U／g的植物有43种，高于2000U／g的植物有14种；在61科植物中，以槭树科和壳斗科植物叶中的蛋白酶活性较高；叶中蛋白酶活性最高的植物是元宝槭和红果罗浮槭，叶中蛋白酶活性分别为5228U／g和5092U／g，与菠萝果的蛋白酶活性（6184U／g）较为接近，具有很高的研究和开发价值。

To find new plant protease,the plant leaf protease activity（LPA） of 190 species belonging to 61 families in subtropical zone were determined by improved Kunit mensuration. The LPA of 133 species were less than 1 000 U/g,the LPA of 43 species were 1 000 -2 000 U/g,and the LPA of 14 species were higher than 2 000 U/g. LPA of Aceraceae and Fagaceae were higher than other families＇, and the LPA of Acer truncaturn and Acer fabri vat. rubrocarpum were the highest, being respectively 5 228 U/g and 5 092 U/g,approxlmate to that of bromelain（6 184 U/g）.