氧化葡萄糖酸杆菌原生质体的形成和再生研究

Protoplast Formation and Regeneration of Gluconobacter Oxydans

报道氧化葡萄糖酸杆菌原生质体的形成和再生的方法。将最佳生长条件下对数生长后期的培养物以溶菌酶处理,YE培养基上再生原生质体、原生质体形成率约99%,再生率2.93%。

Protoplast formation and regeneration of Gluconobacter oxydans G10-3 were reported. The late exponential stage culture under optimum growing conditions was treated with lysozyme in hypertonic buffer. Protoplast was regenerated successfully on YE medium, and the formation and regeneration rates were 99% and 2. 93% respectively.