用放射免疫沉淀试验检查几种感染鼠疫菌的试验动物F1抗体

Detection of F1 Antibody by RIP in Several Kinds of Experinental Animals Infected with Y. pestis

用RIP对感染鼠疫菌的黄胸鼠等5种鼠类血清和脏器进行测定,结果表明脏器可检出F1体:一般情况,检出菌及IHA阳性标本难于查出抗体,而检出F1抗体者又难于培养出鼠疫菌:血清抗体阳性率及滴度与鼠疫菌攻击量无相关关系而与感染率有一定关系。

Sera and viscera of rats, including R. flavipectus etc., infected with Y. pestis, were examined by RIP, results showed that Fl antibody could be detected in viscera. It was also found that Fl antibody was rarely detected in most of the IHA positive specimens from whichY. pestis was isolated and, from Fl antibody positive specimens Y. pestis could be hardly isolated; and that serum antibody positive rate and titres did not correlated with the challenge dose of Y. pestis, however, they were, to some extent, relative to infection rate.