牛布鲁氏菌病诊断中应用EDTA试验的比较及评价

Comparison and Assessment of the EDTA Seroagglutination Test for the Diagnosis of Bovine Brucellosis

本文报告了诊断牛布鲁氏菌病一种新的EDTA改良试验、作者用此法对不同来源的牛血清进行检测,结果表明EDTA试验明显地降低了326份健康牛血清中非特异性凝集滴度、但对布鲁氏菌感染的10份病牛血清凝集滴度没有大的改变。不论SAT还是EDTA试验结果完全一致,而且SAT和CFT结果也相符,在SAT单项诊断中可疑和阳性的139头牛中有98头(70.5%)用EDTA试验证实为假阳性反应试验中还将血清中各类Ig进行EDTA敏感性检查,发现对EDTA不稳定的凝集素主要是IgM。本试验操作简便,能提高牛布鲁氏菌病正确诊断率。

Bovine sera of different sources were tested using FDTA modified scrum and antigen for the diagnosis of bovine brucellosis, results showed that, in the test with 326 sera of healthy cattle. titres of non-specific agglutinations were greatly reduced while in the 10 sera of cattle with Brucella infections, agglutination titres were only slightly altered. Results obtained by SAT were identical to those by EDTA and results by SAT were coincident with those by CFT. Ninty-eight (70.5%) of the 139 sera which showed suspectahle or positive results by SAT were demonstrated by EDTA test to be false positive reactions. All classes of immunoglobulins were examined by EDTA for their sensitivities, it was found that IgM was the main Ig unstable to EDTA. Our study suggested that the FDTA seroagglutination testwas a simple method which could improve the diagnosis of bovine brucellosis.