胃宁方对人胃癌SGC-7910细胞增殖凋亡的影响

Effects of “Weining Decoction” on Proliferation and Apoptosis of Human Gastric Cancer SGC-7910 Cells

目的观察胃宁方对人胃癌SGC-7910细胞增殖和凋亡的影响,并探讨其可能的作用机制。方法不同浓度不同时间的胃宁方处理人胃癌SGC-7910细胞,MTT法测定细胞增殖情况,流式细胞仪分析细胞周期、凋亡及增殖细胞核抗原(PCNA)、c-Myc、Bcl-2表达。结果胃宁方能抑制SGC-7910细胞增殖,抑制率与浓度呈依赖关系;细胞周期G0/G1期、S期比例增加,胃宁方能诱导细胞凋亡,凋亡率与时间、剂量呈依赖关系;胃宁方作用后PCNA、c-Myc、Bcl-2表达显著下调(P<0.05或P<0.01)。结论胃宁方能抑制SGC-7910细胞增殖,诱导其凋亡;其作用机制可能与下调PCNA、c-Myc、Bcl-2的表达有关。

Objective To study the effects of ＂Weining Decoction＂ on proliferation and apoptosis of human gastric canrcinoma SGC- 7910 cells and explore its possible mechanism. Methods Human gastric canrcinoma SGC-7910 cells were treated with ＂Weining Decoction＂ at varied concentrations and times. Then the proliferation of cells was detected by MTT method. Apoptosis,cell cycle and proliferating cell nuclear antigen （PCNA）,c-Myc and Bcl-2 were determined by flow cytometry. Results ＂Weining Decoction＂ could inhibit the proliferation of SGC-7910 cells as direct relation of concentrations. The percent of G0/G1 and S phase cells is increased. The expression of PCNA,c-Myc and Bcl-2 were obviously declined（P〈 0.05 or P〈 0.01）. Conclusion ＂Weining Decoction＂ can inhibit the proliferation and promote the apoptosis of SGC-7910 cells possibly by declining the expression of PCNA, c-Myc and Bcl-2.