葡萄幼胚培养技术的研究(Ⅰ)——激素对胚培养的影响及培养基的筛选

The Study on the Technique of Embryo Culture of Grapes(Ⅰ)

选用巨峰、里扎马特和无核白葡萄的幼胚接种在附加了GA3、IAA、6-BA等的不同激素水平的MS培养基上进行培养,结果表明花后60d的巨峰和花后50d的无核白的幼胚在附加GA30.5mg.L-1、IAA2.0mg.L-1、6-BA0.5mg.L-1的培养基上分别得到了44.0±8.9%和96.0±8.9%的发育率与31.9±6.4%和37.5%的成苗率;花后60d的里扎马特幼胚在附加GA30.5mg.L-1、IAA0.5mg.L-1、6-BA0.5mg.L-1的培养基上得到了76.0±8.9%的发育率和63.5±5.5%的成苗率.筛选出了适合不同品种的最佳胚培养、萌发以及成苗培养基,为葡萄新品种的选育提供了新的方法与技术依据.

Young embryos of Thompson Seedless （v. vinifera cv. Sultanina） grapes of the 50 days after florescence, and Kyoho （V. viniferaxV, labrusce cv.）, Rizamat （V. vinifera cv.） grapes of the 60 days after florescence were cuhured on MS medium supplemented with different concentrations of GA3, IAA and 6-BA in order to study the techniques of embryo culture. The results indicated that the best media of inoculation of embryos of Thompson Seedless, Kyoho and Rizamat grapes were selected. We got 44±8.9%, 96.0±8.9% development rate and 31.9±6.4%, 37.5% germinating rate （p〈0.01） of Thompson Seedless＇ and Kyoho＇ s embryo in the medium with MS＋GA3 0.5mg·L^-1＋ IAA 2.0mg·L^-1 ＋ 6-BA 0.5mg·L^-1, 76.0±8.9% development rate and 63.5±5.4% germinating rate （p 〈 0.01） of Rizamat＇ s embryos in the medium with MS ＋ GA3 0.5mg ·L^-1＋ IAA 0.5mg·L^-1 ＋6-BA 0.5mg·L^-1.