ELISA双McAb夹心法检测鼠疫F1抗原

F1 Antigen Detection By Double McAb Sandwich ELTSA

对应于同一F1抗原分子且不相互阻断的2株F1 McAb,分别制备酶标抗体并包被反应板,用于ELISA双McAb夹心法检测鼠疫F1抗原。以PcAb-RIHA和四步检查为对照,检测不含F1抗原的细菌8株,非鼠疫疫区8种动物的新鲜和腐败标本723份,检测结果全部阴性,和四步检查结果相同,PcAb-RIHA出现非特异反应45份。检测鼠疫强毒菌18株和感染鼠疫标本27份,GMT比PcAb-RIHA高2~4和2~3。本法特异性、敏感性、试剂稳定性均高于PcAb-RIHA。应用于鼠疫疫源地调查、疫情监测、人间疫情判定、细菌学研究效果良好。

Microtiter plates were coated with 2 enzyme-labelled Fl-McAb lines to the identical Fl antigens respectively, to establish the double McAb sandwich ELFSA for the detection of Flantigen and, results were compared with those by PcAb-RIHA and routine method. While PcAb-RIHA had nonspecific reactions in 45 of the specimens, both double McAb sandwich ELISA and the routine method showed identical negative reactions when 8 strains of bacteria without Fl antigen and 723 fresh or putrid specimens from 8 kinds of non-plague animals were tested. And GMT values by the ELISA were 24 and 23 higher than those by PcAb-RIHA, respectively in the examination of 18 strains of strongly virulent Y. pestis and 27 plague specimens. Double Fl-McAb sandwich ELISA is, therefore, better than PcAb-RIHA in specificity, sensitivity and stability; it can be effectively applied to the investigation, surveillance and diagnosis of plague.