冷冻保存异体显微静脉重建血循环实验研究

EXPERIMENTAL STUDY OF CRYOPRESERVED ALLOGENIC MICROVEIN GRAFTS IN RECONSTRUCTION OF BLOOD CIRCULATION/

实验旨在探讨临床应用冷冻保存异体显微静脉，重建血循环的可能性。以新鲜自体静脉移植为对照，将二步冷冻保存家兔股静脉直径１．０～１．４ｍｍ，异体移植修复股静脉缺损。通过光镜、电镜及血管造影检查，观察静脉保存及移植后组织病理学改变和不同时期通畅率。结果证明，静脉内皮细胞超微结构保存良好，移植后排斥反应弱，通畅率：１周９０％，１２周８５％，与对照组无差异（Ｐ＞０．０５）。冷冻保存异体显微静脉有可能作为静脉替代物应用于临床。

In order to determine the fasibility of reestablishment of circulation with cryopreserved microvenous allografts (1.0～1.4mm in diameter), 40 rabbits were divided into 2 groups. In the control group, the fresh autografts were used. In the experimental group, 20 rabbits femoral vein segments were treated by a two step freezing procedure. After stored in liquid nitrogen for 48 hours, the segments were implanted into the femoral veins as allografts. The histological as well as the pathological studies were performed with light and electron microscope, and its patency was determined by angiography. The results showed that the preservation of vein was generally good. The rejective response was weak. The patency rates of 1 week and 12 weeks were 90% and 85% respectively, and there was no significant difference with that of the allogenic fresh autografts (P>0.05). It was suggested that clinical use of cryoperserved allogenic microvein grafts instead of fresh autografts was possible.