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甲状腺转录因子-1及相关蛋白26对SP-B基因表达的调控作用 被引量：2

Role of the TTF-1 and Its Associated Protein 26 in the Regulation of Surfactant Protein-B Expression
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摘要 甲状腺转录因子-1(thyroidtranscriptionfactor-1,TTF-1)是调节SP-B基因表达的关键因子。最近研究发现了一种新的蛋白即TTF-1相关蛋白(TAP26),它不仅能与SP-B基因启动子近侧序列相互作用,而且还能与TTF-1形成蛋白复合物,促进SP-B基因的表达。采用染色质免疫沉淀分析证实TAP26是肺细胞中SP-B启动子转录复合物的一个组成部分,为SP-B基因表达的调控提供分子机制。 Thyroid transcription factor-1 （TTF-1） is a known key factor that controls the gene expression of surfactant protein-B （SP-B）. Recently a novel transfactor, a 26 ku protein was identified and now designated as TTF-1-associated protein 26 （TAP26）. It interacts with the SP-B promoter fragment, forms a protein complex with TTF-1 in vitro and is capable of enhancing human SPB promoter activity in vivo. Using the chromatin immunoprecipitation assay （CHIP）, it was demonstrated that TAP26 was a component of the transcription complexes of the SP-B promoter. It is a newly found molecular mechanism for the regulation of SP-B expression.
作者 柴新群 邓飞涛
机构地区 华中科技大学同济医学院附属协和医院肝胆外科 华中科技大学同济医学院附属协和医院妇产科
出处 《医学分子生物学杂志》 CAS CSCD 2006年第5期368-370,共3页 Journal of Medical Molecular Biology
关键词 甲状腺转录因子-1 TTF-1相关蛋白26 表面活性蛋白-B TtF-1 TAP26 SP-B
分类号 Q344.14 [生物学—遗传学]
  • 相关文献

参考文献14

  • 1[1]MARGANA R K,BOGGARAM V.Functional analysis of surfactant protein B (SP-B) promoter:sp1,Sp3,TTF-1,and HNF-3alpha transcription factors are necessary for lung cell-specific activation of SP-B gene transcription[J].J Biol Chem,1997,272:3083 -3090.
  • 2[2]YANG M C,GUO Y,LIU C C,et al.The TTF-1/TAP26 complex differentially fzsw @ mails.tjmu.edu.cnmodulates surfactant protein-B (SP-B) and -C (SP-C) promoters in lung cells[J].Biochem Biophys Res Commun,2006,344:484-490.
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同被引文献11

  • 1MARGAN R K, BOGGARAM V. Functional analysis of surfactant protein B (SP-B) promoter: Sp1, Sp3, TTF-1, and HNF-3alpha transcription factors are necessary for lung cell- specific activation of SP-B gene transcription[J]. J Biol Chem, 1997,272(5) :3083-3090.
  • 2YANG Y S, YANG M C, WANG B, et al. BR22, a novel protein, interacts with thyroid transcription factor-1 and activates the human surfactant protein B promoter[J]. Am J Respir Cell Mol Biol, 2001,24 (1) : 30-37.
  • 3YANG M C, WANG B, WEISSLER L C, et al. BR22, a 26 kDa thyroid transcription factor-1 associated protein (TAP26), is expressed in human lung cells[J]. Eur Respir J, 2003,22(1) : 28-34.
  • 4LI J, GAO E, MENDELSON C R. Cyclic AMP-responsive expression of the surfactant protein-A gene is mediated by increased DNA binding and transcriptional activity of thyroid transcription factor 1[J]. J Biol Chem, 1998,273 (8): 4592- 4600.
  • 5BOUSQUET W J. Understanding the regulation of surfaetant gene expression[J]. Eur Respir J, 2003,22(1) : 6-7.
  • 6YANG M C, GUO Y, LIU C C, et al. The TTF-1/TAP26 complex differentially modulates surfactant protein-B (SP-B) and-C (SP C) promoters in lung cells[J]. Biochem Biophys Res Commun, 2006,344 (2) : 484-490.
  • 7YANG Y S, YANG M C, WANG B, et al. BR22, a novel protein, interacts with thyroid transcription factor-I and activates the human surfactant protein B promoter[ J ]. Am J Respir Cell Mol Biol,2001,24( 1 ) :30-37.
  • 8YANG M C,WANG B, WEISSLER L C,et al. BR22,a 26 kDa thyroid transcription factor-1 associated protein (TAP26) ,is expressed in human lung cells[ J]. Eur Re- spir J,2003,22( 1 ) :28-34.
  • 9LI J, GAO E, MENDELSON C R. Cyclic AMP-responsive expression of the surfactant proteinA gene is mediated by increased DNA binding and transcriptional activity of thy- roid transcription factor-1 [ J ]. J Biol Chem, 1998,273 ( 8 ) :4592-4600.
  • 10FISHER C L,PEI G K. Modification of a PCR-based site-directed mutagenesis method[ Jj. Biotechniques, 1997,23 (4) :570-574.

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医学分子生物学杂志
2006年 第5期

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