六味地黄颗粒协同辅舒酮吸入对哮喘大鼠IFN-γ和IL-4影响的研究

Effect of Liuweidihuang Granula Cooperating with Flixotide Aerosol on IFN-γ and IL-4 of Asthma Rat Models

目的:研究滋阴补肾代表方六味地黄颗粒协同辅舒酮吸入对大鼠哮喘模型过敏性气道炎症的作用。方法:Wistar大鼠随机分成6组,除正常动物对照组外,其余各组均以卵蛋白(OVA)辅以氢氧化铝为佐剂注射致敏,2周后雾化吸入卵蛋白激发哮喘,建立哮喘模型。从实验第8天开始,给药组辅舒酮喷雾给药,六味地黄颗粒灌胃给药,正常组和模型组灌服生理盐水。正常组用生理盐水替代卵蛋白进行注射和吸入。结果:六味地黄颗粒加不同剂量辅舒酮组大鼠肺泡灌洗液中γ-干扰素(IFN-γ)水平及IFN-γ/IL-4比值明显高于模型组(P<0.01,P<0.05)。结论:六味地黄颗粒协同辅舒酮吸入具有减轻哮喘气道慢性炎症的作用。

Objective： To observe the effect of representative formula of nourishing yin and invigorating the kidney （ Liuweidihuang Granula） cooperating with Flixotide aerosol inhalation on the allergic airway inflammation of asthma rat models. Methods： Wistar rats were randomly divided into six groups. Except the normal control group, all the other 5 groups were sensitized by injecting into the abdominal cavity of the ovalbumin （OVA） together with aluminum hydroxide as adjuvants, 2 weeks later followed by aerosolized OVA challenge to prepare the asthma models. From the eighth day of the experiment, in medication administration teams, rats were sprayed with Flixotide aerosol and given intrngastrie administration with Liuweidihuang Granula. The normal control group and the asthma model group were drenched with normal saline. Results： There was no difference between the groups given Liuweidihuang Granula and different dosages of Flixotide aerosol but the amount of IFN-γ and IFN-γ/IL-4 in BALF in the Liuweidihuang Granula cooperating with Flixotide aerosol group were higher than that in the model group （ P 〈 0.01, P 〈 0.05 respectively）. Conclusion： Liuweidihuang Granula cooperating with Flixotide aerosol may inhibit airway inflammation in rats with asthma.