酸性复合消毒剂杀菌效果及其毒性试验观察

EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY AND TOXICITY OF AN ACIDIC COMPOUND DISINFECTANT

酸性复合消毒剂主要杀菌成分为过氧乙酸与过氧化氢,为观察其杀菌效果及其毒性,采用悬液定量杀菌试验和动物试验方法进行了实验室研究。结果,以含过氧化氢300 mg/L与过氧乙酸50 mg/L的复合消毒液对悬液中大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌作用2.5 m in,杀灭对数值均>5.00;含过氧化氢12 g/L与过氧乙酸2 g/L的复合消毒液对悬液中枯草杆菌黑色变种芽孢作用5.0 m in,杀灭对数值>5.00。该消毒剂对小鼠急性经口毒性LD50>5000 mg/kg(体重),属无毒类;小鼠骨髓红细胞微核试验为阴性;含过氧化氢300 mg/L与过氧乙酸50 mg/L的复合消毒液对家兔皮肤无刺激性。结论,酸性复合消毒剂具有很好的杀灭细菌繁殖体和细菌芽孢的效果,属于无毒类物质。

The acidic compound disinfectant contains peracetic acid and hydrogen peroxide as the main germicidal ingredients. In order to observe its germicidal efficacy and toxicity, suspension quantitative germicidal test and animal test method were used to carry out laboratory study. Results： The killing logarithm values of Escherichia coil, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans in suspension exposed to the compound disinfectant solution containing hydrogen peroxide 300 mg/L and peracetic acid 50 mg/l, for 2.5 min were 〉5.00 and that of spores of Bacillus subtilis vat. niger in suspension exposed to the compound disinfectant solution containing hydrogen peroxide 12 g/L and peracetic acid 2 g/L for 5.0 min was 〉5.00. The mouse acute oral toxicity of the disinfectant was LD50 〉5000 mg/kg（ bw）, belonging to non - toxic class. The result of mouse bone marrow erythrocyte micronucleus test was negative. The compound disinfectant solution containing hydrogen peroxide 300 mg/L and peracetic acid 50 mg/L was non - irritating to rabbit skin. Conclusion ： The acidic compound disinfectant has excellent efficacy in killing vegetative form and spore of bacteria and belongs to non - toxic substance.