ABC-ELISA技术用于检测脑脊液囊虫抗体的研究

The Application of ABC-ELISA to the Detection of Cysticercus Antibody in Cerebrospinal Fluid

本文应用生物素—亲和素系统(BAS)的Avidin/Biotin-HRP Complex(ABC)法,对21例脑囊虫病与65例非胶囊虫病患者脑脊液中抗囊虫抗体进行了检测,并与常规ELISA比较。结果表明,ABC-ELISA法阳性率为90.5％,常规ELISA法为81.0％;抗体几何平均滴度分别为1:66.66和1:28.87。两法的抗体滴度差异有显著性(P<0.05)。ABC-ELISA的敏感性相当于常规ELISA的2.3倍。有2例ABC法阳性而常规ELISA阴性,这说明ABC-ELISA敏感性高,并可减少常规ELISA的假阴性,在抗体水平低的患者尤为敏感。非脑囊虫病人脑脊液两法均为阴性,表明ABC-ELISA法为脑囊虫病的免疫诊断提供了一种比常规ELISA更敏感的新方法。

ABC-ELISA was used in the detection of Cysticercus antibodies in cerebrospinal fluid (CSF) of 21 cerebral-cysticercosis patients with 65 non-cysticercosis patients as controls, and the results were compared with those by conventional ELISA. Results showed that antibody positive rate by ABC-ELISA was 90.5% while that of conventional ELISA was 81.0%. GMTs for both methods were 1: 66.66 (ABC-ELISA) and 1:28.87 (ELISA), respectively; with significant difference (P<0.05) and, sensitivity of ABC-ELISA was 2.3 times as high as that of ELISA. Two cases which were antibody positive by ABC-ELISA were negative by ELISA, indicating that the former had higher sensitivity. No false positive reaction was observed in CSF of the 65 non-cysticercosis patients by either method. The present results indicated that ABC-ELISA, with a higher sensitivity and unaltered specificity, was very useful for the diagnosis of cerebral cysticercosis, especially for the identification of cases with lower immune responsiveness.