Cu(phen)_2^(2+)与6-羟基嘌呤及DNA的相互作用

The Interaction among Cu(phen)_2^(2+),6-Mercaptopurine and DNA

目的为开发新的抗癌药物提供一定的理论研究依据。方法在Tris-NaCl(pH=7．2)缓冲溶液中,应用电化学实验、电子吸收光谱分析等技术研究了Cu(phen)_2^(2+)(phen=1,10-邻菲咯啉)与6-羟基嘌呤(6-HX)及DNA间的相互作用。结果Cu(phen)_2^(2+)与6-HX发生了较弱的相互作用,其作用产物与DNA间也存在较弱的相互作用。结论在此作用体系中Cu(phen)_2^(+2)、6-HX、Cu(phen)_2^(2+)·6-HX复合物间可能存在如下平衡：Cu (phen)_2^(2+)+6-HX(?)Cu(phen)_2(2+)·6-HX复合物,少量DNA的存在可以加速Cu(phen)_2^(2+)·6-HX复合物的解离。

Objective ： To provide the theoretical basis for developing new anticancer drug. Methods ： The interaction of Cu（ pben）2^2＋（pben = 1,10-pbenantbroline） with 6-bypoxantbine （6-HX） and DNA in Tris-NaCl buffer solution （pH = 7. 2）was investigated by electrochemistry, electron absorption spectroscopy technique. Results：Experimental results showed a weak interaction between Cu（ phen）2^2＋ complex and 6-HX, and the interaction of their binding product with calf thymus DNA was also weak. Conclusion ：The results indicate that there is an obvious interaction equilibrium among Cu（ phen）2^2＋ complex, 6-HX and DNA as follows： Cu（ phen）2^2＋＋ 6-HX ←→Cu（ phen）2^2＋·6-HX. When DNA is added to the solution, it makes the interaction equilibrium move leftwards.