光氧化反应处理牛颈静脉带瓣管道的理化性能与钙化实验研究

Physiochemical propertities and experimental study of calcification of bovine jugular conduit with valves stabilized by dye-mediated photo-oxidation

目的通过光敏剂介导的光氧化反应处理牛颈静脉带瓣管道,观察其理化与抗钙化性能。方法新鲜牛颈静脉带瓣管道24根,随机分为3组：光氧化反处理组(Ⅰ组)；戊二醛处理组(Ⅱ组)；新鲜对照组(Ⅲ组),不作固定处理。3组分别测组织含水量、最大应力值、组织蛋白含量；前两组植入活体1月后测组织含钙量。结果与Ⅲ组相比,Ⅰ组和Ⅱ组的管道组织蛋白含量都明显减少(P＜0．05),最大应力值均明显提高；组织含水量方面,Ⅰ组与Ⅲ组无差异,Ⅱ组明显减低(P＜0．05)；但钙化程度上,Ⅰ组显著低于Ⅱ组(P＜0．001)。结论经光氧化反应处理的牛颈静脉带瓣管道特性更接近于新鲜组织,但又同戊二醛处理的管道一样,具有组织稳定性,且钙化程度低,可望成为一种好的临床生物材料。

Objective： To observe the physiochemical properties and calcification resistance of the bovine jugular conduits with valves stabilized by dye-mediated photo-oxidation Methods ： Twenty-four bovine jugular conduits with valves were divided into 3 groups and treated with dye-mediated photo-oxidation （ Group Ⅰ ）, glutaraldehyde （ Group Ⅱ ） and untreated group （ Group Ⅲ） respectively. Tissue water content , breaching strength and tissue protein content were studied. Pieces of Ⅰ and Ⅱ groups were implanted in back of SD rats. 30 days later, calcium concent analysis was carried out. Results： There was no difference in tissue water content between Group Ⅰ and Ⅲ, but there was significant difference between Group Ⅰ and Ⅱ. the breaching strength of Group Ⅰ was higher than that of Group Ⅲ（ P 〈 0.05 ）, but lower than that of Group Ⅱ （P 〈 0.05 ）. A decrease in tissue protein content was found in Group Ⅰ and Ⅱ. The tissue calcium content of Group Ⅰ was obviously lower than that of Group Ⅱ （ P 〈 0. 001 ）. Conclusions ： The dye - mediated photo-oxidation can effectively preserve the tissue stability and reduce the calcification.