血府逐瘀汤对血瘀证大鼠血管内皮细胞黏附分子表达的影响

Effects of Xuefu Zhuyu decoction on the expression of adhesion molecules in vascular endothelial cells of blood stasis rat

目的:研究血府逐瘀汤对血瘀证血管内皮细胞黏附分子表达的影响。方法:采用免疫组织化学和RT-PCR两种方法观察血府逐瘀汤不同剂量(高、中、低)对血瘀证(大鼠)血管内皮细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)、血小板-内皮细胞黏附分子-1(PECAM-1)和诱生型一氧化氮合酶(iNOS)表达的影响。结果:模型组ICAM-1、VCAM-1、PECAM-1、iNOS高表达,血府逐瘀汤能减少造模动物ICAM-1、VCAM-1、PECAM-1、iNOS的表达,而且随着药物剂量的减少,各分子表达呈递增趋势,具有量效关系。结论:血府逐瘀汤能降低血瘀证大鼠血管内皮细胞黏附分子表达,且量效关系明显。

Objective：To study the effects of Xuefu Zhuyu decoction on the expression of adhesion molecules in vascular endothelial cells（VECs） of blood stasis rats. Methods： The expressions of VCAM-1, ICAM-1, PECAM-1, iNOS and their corresponding mRNA in VECs were assayed with the methods of immunohistochemistry, image analysis and RT- PCR under the function of different Xuefu Zhuyu decoction doses（high, middle, low）. Results： Immunohistochemistry and image analysis indicated： the expressions of VCAM-1, ICAM-1, PECAM-1 and iNOS were higher in the model group than in the control group, Xuefu Zhuyu decoction could decrease the expression of VCAM-1, ICAM-1, PECAM-1 and iNOS in the model group, moreover as the dose decreases, the expression of these molecules increase, it was of dose-effect relation. Conclusions： Xuefu Zhuyu decoction can decrease the expression of adhesion molecules in vascular endothelial cells of blood stasis rats,and the dose-effect relation is obvious.