抗体阳性MS患者3种IFNβ诱导基因表达的多样性分析

Multiplex analysis of expression of three IFNβ-induced genes in antibody-positive MS patients

Some interferon beta (IFNβ)-treated patients with multiple sclerosis develop antibody-mediated decreased bioactivity with resultant loss of therapeutic effect. The authors developed realtime multiplex reverse transcriptase PCR to measure expression of three IFNβ-inducible genes to directly assess IFNβbioactivity in patients with neutralizing antibodies (NAbs). The three genes responded in tandem. Correlation of NAb level with bioactivity at low/ moderate NAb levels was poor, indicating that for such patients, direct measurement of IFNβbioactivity is most reliable.

Some interferon beta （IFNβ）-treated patients with multiple sclerosis develop antibody-mediated decreased bioactivity with resultant loss of therapeutic effect. The authors developed reahime multiplex reverse transcriptase PCR to measure expression of three IFNβ-inducible genes to directly assess IFNβ bioactivity in patients with neutralizing antibodies （NAbs）. The three genes responded in tandem. Correlation of NAb level with bioactivity at low/ moderate NAb levels was poor, indicating that for such patients, direct measurement of IFNβ bioactivity is most reliable.