高效液相色谱法测定小儿复方磺胺甲噁唑颗粒中两组分含量

Determination of Trimethoprim and Sulfamethoxazole in Pediatric Compound Sulfamethoxazole Granules by HPLC

目的建立测定小儿复方磺胺甲噁唑颗粒中磺胺甲噁唑和甲氧苄啶含量的方法。方法高效液相色谱法;采用DiamonsalC18柱(200mm×4.6mm,5μm)为色谱柱,检测波长为246nm,以0.025mol/L磷酸溶液(用三乙胺调节pH=3.0)-乙腈(79∶21)为流动相,流速为1mL/min。结果磺胺甲噁唑和甲氧苄啶可良好分离;磺胺甲噁唑的浓度线性范围为0.4～4μg/mL,r=1.0000,平均回收率为100.05%,RSD=0.24%;甲氧苄啶的浓度线性范围为0.08～0.8μg/mL,r=0.9996,平均回收率为100.17%,RSD=0.35%。结论该法操作简单、快速,结果准确,两组分的分离效果好。

Objective To establish a HPLC method for the determination of trimethoprim and sulfamethoxazole in pediatric compound sulfamethoxazole granules. Methods A Diamonsal C18 column was used with 0.025 mol/L phosphoric acid （adjusted pH to 3.0 with triethylamide buffer）-acetonitrile（79 ： 21） as mobile phase. The flow rate was 1 mL/min and with wavelength of 246 nm. Results The linear range was 0.4 - 4μg/mL for trimethoprim with r = 1.000 0 and 0.08 - 0.8μg/mL for sulfamethoxazole with r = 0.999 6. The average recovery rate was 100. 05% , RSD =0.24% for trimethoprim and 100. 17% , RSD = 0. 35% for sulfamethoxazole. Conclusion The method is simple and accurate. Trimethoprim and sulfamethoxazole can be determined well.