双抗胶原膜的临床抗菌抗降解观察

Observation of clinical antibacteria and anticollagenase activities of bianticollagen membrane

目的：评估自行研制用于引导牙周组织再生（ＧＴＲ）的双抗胶原膜（ＢＡＣＭ）在临床应用中实际抗菌能力和抗酶解性能。方法：对１２例牙周炎患者行体内药物释放试验，以高效液相色谱测其龈沟液四环素含量；应用扫描电镜观察ＧＴＲ术后１周取出的ＢＡＣＭ膜上的细菌附着情况。结果：牙周袋内放置四环素１５０μｇ，１周后龈沟液中药物浓度为２０．７８μｇ／ｍｌ，扫描电镜观察胶原膜纤维结构完好无损，ＢＡＣＭ上细菌数明显少于对照膜（ＧＬＣＭ）。结论：临床评估ＢＡＣＭ能显著减少细菌对胶原膜的附着，并具有一定抗胶原酶的性能。

Aim:To prepare bianticollagen menbrane(BACM) for guided tissue regeneration (GTR) and to evaluate efficiency of antibacteria and anticollagenase activities of BACM in clinic.Methods:Tetracycline membrane (150μg) was placed into pocket of 12 patients with periodontitis, after one week the concentration of tetracycline in gingival crevicular fluid( GCF) was detected by high performance liquid chromatograph.BACM and control membrane (GLCM) from periodontal site one week after undergoing guided tissue regeneration (GTR) technique were observated by scanning electromicroscopy (SEM). Results:The concentration of tetracycline in GCF was 20.78μg/ml.SEM showed that BACM construction was complete and colonial bacteria on BACM was much less than that on GLCM.Conclusions: BACM had clinic efficiency of antibacteria and anticollagenase activities in GTR.