多重耐药铜绿假单胞菌产超广谱β-内酰胺酶基因研究

Extended Spectrum β-Lactamases Genes in Multidrug-resistant Pseudomonas aeruginosa

目的调查多重耐药铜绿假单胞菌(PAE)产超广谱β-内酰胺酶(ESBLs)基因的流行状况。方法采用琼脂稀释法对35株临床分离的PAE进行抗菌药物敏感试验,PCR方法检测7种ESBLs基因。结果35株PAETEM、OXA、PER、GES的阳性率分别为51.4%、42.8%、31.4%、22.9%,而SHV、VEB、CTX-M-1基因均阴性。结论我院临床分离PAETEM、OXA、PER、GES基因携带率高,检出GES基因为国内首次报道。

OBJECTIVE To investigate the situation of prevalence for extended spectrum β-lactamases（ESBLs）genes in multidrug-resistant Pseudomonas aeruginosa （PA）, METHODS Antimicrobial susceptibility test was performed by agar dilution method and 7 kinds of ESBLs genes were detected by PCR methods in 35 strains of PA. RESULTS In 35 strains of PA, the positive rates of genes of TEM, OXA, PER and GES were 51.4%, 42.8%, 31, 4%, and 22.9%, respectively, and genes of SHV, VEB, and CTX-M-1 were all negative. CONCLUSIONS This study shows that there are high positive rates of TEM, OXA, PER and GES genes in PA in our hospital. The GES genes in PA are first reported in China.