肝炎患者肝组织内纤连蛋白表达变化的研究

Expression of Fibronectin in Hepatic Tissues from Patients with Hepatitis

目的研究纤连蛋白(FN)在不同类型的肝炎和肝硬化患者肝组织中的表达和分布及其与血清透明质酸(HA)水平的关系。方法用免疫组化方法和常规病理技术确定纤连蛋白在正常和病变肝组织内的定位和表达量的变化规律,并以放射免疫学方法检测肝病患者血清内透明质酸的含量。结果慢性活动性肝组织中肝细胞和内皮细胞内FN的阳性细胞率明显高于急性肝炎(AH)组和慢性迁延性肝炎(CPH)组(P<0.05);FN在肝硬化患者肝细胞内不表达,在内皮细胞内呈弱阳性表达,但在各型肝炎的炎症细胞浸润区内FN呈阳性表达;肝组织中FN阳性表达的患者血清内HA含量为(203.7±121.6)ng/ml,明显高于阴性和弱阳性表达患者(139.2±158.0)ng/ml和(130.4±65.1)ng/ml(P<0.01)。结论FN是肝纤维化的前驱物质,参与新生纤维组织的形成,与肝组织的损伤及其修复和血清内HA含量增大有关。

OBJECTIVE To examine expression and distribution of fibronectin （FN） in hepatic tissues from patients with hepatitis or cirrhosis, and its correlation with serum hyaluronic acid （HA） contents. METHODS The expression and distribution of FN were detected with immunohistochemical and pathological methods in liver tissues of 66 cases, among which there were 8 cases of normal liver tissues, 58 cases of various types of hepatitis. The levels of HA in patients with liver disease were detected with radioimmunological method. RESULTS Positive cellular rate of FN in hepatocytes and endothelial cells of chronic active hepatitis （CAH） was apparently higher than those in acute hepatitis （AH） group and chrouic persistent hepatitis （CPH） group（P〈0.05）. FN was not expressed in hepatocytes and weakly expressed in endothelial ceils from patients with hepatic cirrhosis （HC）. FN was positively stained in inflammatory cell infiltrating lesions of various types of hepatitis. The level of serum HA was （203.7±121.6）ng/ml in patients with positive FN-staining liver tissues, obviously higher than those in weak or negative FN-staining liver tissues （139.2±158.0）ng/ml and （130.4±65. 1）ng/ml,respectively, （P〈0.01）. CONCLUSIONS FN might be one of the precursor substances of hepatic fibrosis, participating in the formation of fibrous tissues, associated with damage and reparation of hepatic tissues, and increment of serum HA as well.