摘要
目的建立反相高效液相色谱法测定美洛昔康胶囊中美洛昔康的含量。方法选用Waters C18色谱柱,以甲醇-0.2M醋酸铵为流动相,检测波长为362nm,流速0.8ml/min,柱温为室温。结果美洛昔康在0.1-200μg/ml范围内呈很好的线性关系,r=0.9999(n=5),平均回收率为99.2%,RSD为0.39%。结论该法简便、快速、灵敏度高、专属性好,结果准确、可靠,成功用于胶囊制剂中美洛昔康含量的测定。
Objective: To establish a method using reversed-phase high performance liquid chromatography for determination of meloxicam capsules. Method: The column: Waters C18, the mobile phase consists of methanol- 0.2M ammonium acetate, with detection at 362 nm, flow rate 0.8 ml/min and the column temperature at room temperature. Result: The linear range was 0. 1-200μg/ml (r=0. 9999, n=5) with an RSD of 0. 39%. The mean recovery was 99.2%. Conclusion.. This established method is simple, rapid, sensitive, selective and accurate. The method could be successfully applied to the determination of meloxicam in capsules.
出处
《泰山医学院学报》
CAS
2006年第2期97-98,共2页
Journal of Taishan Medical College