三丁基锡对罗非鱼稚鱼生长和ATP酶活力的影响

Effect of Tributyltin Exposure on Growth and Activities of ATPase in Juvenile Tilapia nilotica

在实验生态条件下,研究浓度为0.1、1、10 ng/L的三丁基锡对罗非鱼稚鱼生长、死亡率及Ca2+-ATP酶和Na+,K+-ATP酶活力的影响.结果显示:三丁基锡抑制罗非鱼稚鱼的生长发育.其中15 d的1 ng/L和10 ng/L暴露组体长较对照组显著性降低;15 d和30 d的10 ng/L暴露组死亡率较对照组均显著增加.三丁基锡对罗非鱼稚鱼的Ca2+-ATP酶和Na+,K+-ATP酶活力的抑制均表现剂量依赖性.Ca2+-ATP酶和Na+,K+-ATP酶活力的变化与死亡率具有一定相关性.

To determine whether early life exposure to tributyltin （TBT） impaired postnatal development in fish, the fries of Tilapia nilotica were exposed to environmentally realistic levels （0.1 ng/L, 1 ng/L, 10 ng/L） of TBT for 15 days or 30 days respectively. The effects on growth and mortalities and Ca^2＋-ATPase, Na^＋/K^＋-ATPase activities were evaluated. The results show that the growth of Tilapia nilotica exposed to TBT was inhibited. The total length of Tilapia nilotica exposed to the 1 ng/L or 10ng/L TBT for 15 or 30 days respectively decreased significantly compared to the control. The mortalities of Tilapia nilotica exposed to the 10 ng/L TBT for 15 days or 30 days respectively increased significantly compared to the control. The activities of Ca^2＋-ATPase and Na^＋ ,K^＋-ATPase in all groups were inhibited by TBT in a concentration-related manner compared to the control, the activity of Ca^2＋ -ATPase exposed to the 10ng/L TBT for 15 or 30 days respectively decreased significantly compared to the control. A significant reduction of Na^＋ ,K^＋-ATPase was found when exposed 10 ng/L TBT for 30 days. There is a relationship between Ca^2＋-ATPase, Na^＋/K^＋-ATPase activitives and the morality.