激光诱变株啤酒酿造酵母JY2-2的选育及其中试

Screening of the Brewer′s Yeast Strain JY2-2 by Laser Mutagenesis and Its Pilot Fermentation

用He-Ne激光辐照诱变啤酒酵母(Saccharomyces cerevisiae)XB05,分离抗双乙酰的菌株,通过三角瓶发酵筛选,得到几株优良的菌株,其中菌株JY2-2用500 mL三角瓶装300 mL 12.5°Bx麦芽汁在12℃下发酵8 d,发酵液的发酵度为66%,双乙酰、乙醛和总高级醇的含量分别为0.079 0、7.43和87.22 mg/L.以12°Bx麦芽汁为培养基用500 L发酵罐发酵,发酵7 d发酵液的发酵度为68.8%,双乙酰、乙醛和总高级醇的含量分别为0.034 4、5.12和70.6 mg/L.啤酒口感纯正清爽,突变株JY2-2的主要发酵特性稳定,具有很好的应用前景.

Several fine brewer＇s yeast strains were obtained from the original strain XB05 after mutagenesis with laser and screening. Among the mutants, fermented degree of mutant JY2-2 was 66% ,the content of diacetyl,acetaldehyde and total higher alcohols were respectively 0. 079 0, 7. 43,87.22 mg/L under the fermentation conditions of 500 mL flask with 300 mL 12.5°Bx wort at 12℃ for 8 days. The fermentation degree of mutant JY2-2 was 68.8%, the content of diacetyl, acetaldehyde and total higher alcohols in the fermented liquid were respectively 0. 034 4,70. 6,5.12 mg/L under the conditions of 500 L fermentor with 400L 12°Bx wort at 12℃ for 7 days. The sensory evaluation of the beer produced by the mutant strain JY2-2 was nice. The fermentated properties of the mutant JY2-2 were relatively stable. JY2-2 is of well applied prospect on the beer brewing.