摘要
目的初步探讨卡介苗(Bacillus Calmette-Guerin,BCG)分泌性蛋白在体外培养试验中对人单个核细胞的刺激效应。方法用淋巴细胞转化试验(MTT法)检测单个核细胞经卡介苗纯蛋白衍化物(BCG-PPD)刺激后的增殖程度和IL-2生成情况,同时设ConA对照组。结果BCG-PPD体外刺激人单个核细胞后,单个核细胞增殖程度和IL-2生成量均明显增加,且显著高于有丝分裂原Co-nA对照组(P均<0.001);单个核细胞增殖程度和IL-2生成量之间呈正相关关系(r=0.6 666,P<0.001)。结论BCG分泌性蛋白具有较强的免疫原性,可显著激活单个核细胞功能,提示卡介苗分泌性蛋白在抗结核免疫中起了积极作用。
Objective To observe the influence of Bacillus Calmette-Guerin （BCG）＇s secretory protein to human mononuclear cells （MCs）. Methods The proliferation and IL-2 production of human MCs stimulated with BCG＇s purified protein derivative （BCG-PPD） were detected by lymphocyte transformation test （LTT） and enzyme-linked immunosorbent assay （ELISA）. Results After stimulated with BCG-PPD in vitro, the proliferation and IL-2 production of human MCs were remarkably higher than ConA control group （P〈0. 001）. Significant correlation was showed between the proliferation degree and IL-2 production of human MCs （r=0. 6 666, P〈0. 001）. Conclusion BCG＇s secretory protein has strong immunogenicity can activate human MCs.
出处
《贵州医药》
CAS
2006年第9期777-779,共3页
Guizhou Medical Journal
基金
贵州省高层次人才科研条件特助经费(030516)资助
