光量子加血卟啉疗法对动物移植瘤生长的影响及机制探讨

Effects of light quantum and hematoporphyrin therapy on the growth of tumor in rats

目的:探讨自血光动力学疗法对动物移植瘤生长的影响及机制。方法:本实验选用经腹股沟皮下接种W256瘤细胞株形成实验性荷瘤大鼠40只,采用光动力学疗法对患有移植瘤大鼠进行治疗,并同时检测血浆、肝脏及瘤组织中超氧化物歧化酶的活力及氧自由基代谢产物丙二醛含量。结果:大鼠输入经紫外光照射之血后,其肿瘤生长速度明显减慢(P<0.05),大鼠输入注有血卟啉的血后,其肿瘤生长亦显著减慢(P<0.01);大鼠输入经紫外光照射并含有血卟啉的血后,其肿瘤生长速度比其它组均显著减慢(P<0.01);光照射组大鼠在肿瘤生长减慢同时,其血浆和瘤组织中SOD活力明显升高;MDA含量显著降低(P<0.05,P<0.01);而血卟啉组大鼠其血浆和瘤组织中SOD活力明显降低,MDA含量显著升高(P<0.01);光照射加血卟啉组大鼠,在肿瘤生长减慢同时,其血浆、肝脏及瘤组织中SOD活力较其它组均显著降低,MDA含量显著升高(P<0.01)。结论:经紫外光照射的血或含有血卟啉的血均对肿瘤生长有抑制作用,而且紫外光照射与注入血卟啉相结合对抑制肿瘤生长效果最佳,该光动力学疗法对肿瘤治疗作用机制之一可能是通过产生单态氧而杀伤瘤细胞的。

AIM： To study the effect of photodynamic therapy on the growth of tumor in rats. METHODS： W256 tumor cells were implanted in 40 rats by subcutaneous vaccination at groin. Then, the rats were treated by photodynamic therapy （PDT）. Total saperoxides dismutase （T- SOD ） and malondialdehyde （MDA） in plasma, tumor tissue and liver were detected. RESULTS ： （ 1 ） The growth of tumor was significantly slow after the rats were transfused blood with ultraviolet radiation, with hematoporphyrin or with both ultraviolet radiation and hematoporphyrin （ P 〈0.05, P 〈0.01 ）. （2） The activity of SOD was obviously increased and the contents of MDA were decreased in plasma while the growth of tumor was significantly slow in the ultraviolet radiation group （P 〈0. 05, P 〈0. 01 ）. However, the activity of T - SOD was weakened and the contents of MDA were increased in hematoporphyrin group （P 〈 0. 01 ） and ultraviolet radiation combined with hematoporphyrin group （P 〈 0. 01 ）. CONCLUSIONS： The results indicate that the growth of tumor is inhibited by means of transfusion blood with ultraviolet radiation of hematoporphyrin. The most remarkable inhibitory effect is in ultraviolet radiation and hematoporphyrin group. The generation of singlet oxygen by PDT may be one of the mechanisms of killing tumor cells.