组织因子在肝细胞癌的发生及侵袭转移中的研究

Overexpression of tissue factor levels in plasma and tissue of hepato-cellular carcinoma(HCC)patients and its clinical significance

目的:检测组织因子(TF)在肝细胞癌患者中的表达并探讨其意义。方法:采用酶联免疫法检测肝细胞癌患者50例及对照组30例的血浆TF水平,采用RT-PCR法检测其中27例肝癌、癌旁、正常肝组织TFmRNA表达。结果:①肝细胞癌患者血浆TF显著高于对照组(P<0.05)。血浆TF在分化高低、肿瘤大小及是否合并肝硬化组间表达有显著差异(P<0.05),在有淋巴转移、肝外脏器转移及门脉癌栓组高于无转移及癌栓组(P<0.05),而在不同癌灶数目及包膜情况组间无显著差异(P>0.05)。②TFmRNA在肝癌组织中阳性率及相对表达强度分别为62.96%(17/27)、0.567±0.268,均显著高于癌旁组织及正常组织。阳性患者相对表达强度在肿瘤大小及部分侵袭转移指标中表达有显著差异(P<0.05)。结论:TF在肝癌患者血浆及组织中升高且与部分侵袭转移指标相关,提示其TF可能促进了肝癌的发生与侵袭转移。

AIM： To detect tissue factor （TF） level both in plasma and in tissue of hepatocellular carcinoma （HCC） patients and to elucidate their association with clinical features. METHODS： Plasma TF levels of 50 cases of HCC patients and 30 cases of control were detected by ELISA. 27 HCC tissue samples with their adjacent tissue samples and 27 normal liver tissues were detected by RT - PCR. RESULTS： ① Plasma TF levels were increased significantly in HCC group when compared with control （P 〈 0. 05）. TF levels were higher in poor differentiation, large size and cirrhosis subgroup in HCC patients （ P 〈 0. 05 ）. Plasma TF levels were also significantly increased in extra - hepatic metastasis, lymphatic metastasis and portal venous tumor thrombus subgroups （ P 〈 0. 05 ）. ② The mRNA expression rate of TF in HCC tissue was 62. 96% （17/27） and the relative mRNA expression intensity of TF was 0. 567 ± 0. 268. Those were significantly higher than that in their adjacent tissue samples or 27 normal liver tissue samples （ P 〈 0.05 ）, While the relative expressive intension of TF were also significantly higher in larger size and several invasive and metastatic subgroups （ P 〈 0. 05 ）. CONCLUSION： TF might play an important role in hepatic carcinogenesis, invasiveness and metastasis.