颞叶癫痫大鼠海马差异表达基因和蛋白质的筛选研究

Gene and protein alterations in the hippocampus of rats with temporal lobe epilepsy

目的:寻找颞叶癫痫大鼠海马组织的差异表达基因和蛋白质,以期为进一步探讨颞叶癫痫的发病机制,寻找新的治疗靶点和研发新的治疗手段奠定基础。方法:运用cDNA微阵列、二维电泳和MALDI-TOF-MS技术,分析氯化锂-匹罗卡品(LiCl-PILO)致痫大鼠模型海马组织的基因表达谱和蛋白质表达谱,并对发现的差异表达基因和差异表达蛋白质进行分析和鉴定。结果和结论:发现LiCl-PILO致痫大鼠海马组织中192个基因差异表达,159条可在GenBank中登陆,其中表达上调的基因84条,表达下调的基因75条;筛选到78个差异表达蛋白质斑点,其中31个在癫痫组表达下调,47个在癫痫组表达上调。有5个蛋白质最终鉴定确认。本研究结果为运用蛋白质组学方法寻找癫痫治疗新靶点研究提供实验依据。

AIM： To examine the expression profiles of both genes and proteins in hippocampus of rats with temporal lobe epilepsy （TLE） for revealing the molecular mechanisms of TLE and looking for the candidate targets and new therapeutic approaches in clinical practice. METHODS： Rat temporal lobe epilepsy was induced by administration of lithium chloride and pilocarpine （ LiCl - PILO）. The expression spectra of genes and proteins were constructed through the techniques of cDNA microarray, two- dimensional （2D） electrophoresis and Matrix-assisted laser desorption/ionization timeof- flight mass spectrometry （MALDI -TOF MS）. Subsequently, the differentially expressed genes and proteins were identified and analyzed. RESULTS： There were 192 genes of differential expression observed in hippocampal tissues of LiCI - PILO -induced temporal lobe epilepsy, and 159 genes have been registered in Genbank database, in which 84 genes were up -regulated while 75 genes were down - regulated. 78 protein spots of differential display were screened out, in which 31 proteins were detected to be down - regulated and 47 were up - regulated. Finally, 5 proteins were identified. CONCLUSION： These genes and proteins found in our study may play pivotal roles in the pathogenic mechanisms of epilepsy and may promise new therapeutic targets for refractory epilepsy in the future.