HSP70对大鼠肝脏局部缺血再灌注后炎症反应的影响

Effects of heat shock protein 70 on inflammation after hepatic local ischemia/reperfusion in rats

目的:通过热应激预处理诱导HSP70表达,探讨其对肝脏缺血再灌注损伤后炎症反应的影响。方法:采用大鼠局部缺血再灌注模型(IR组),并在热应激预处理(H+IR组)及槲皮素+热应激预处理(Q+H+IR组)条件下观察肝脏缺血再灌注后HSP70、ICAM-1的表达及MPO的活性;测定血清ALT和AST的活性;电镜观察肝细胞结构的改变。结果:在H+IR组检测的各时点HSP70表达均明显高于其它两组、对肝脏进行缺血再灌注后,肝细胞损伤较轻,血清ALT、AST升高不明显(P<0.01);肝组织中ICAM-1表达增加,以再灌注后6h最显著,MPO活性升高以12h最为显著,但两者变化均低于IR组和Q+H+IR组(P<0.01)。结论:热应激预处理诱导产生HSP70蛋白能够降低大鼠肝脏缺血再灌注损伤过程ICAM-1的表达和MPO活性的改变,进而抑制炎症反应引起的肝脏损伤。

AIM ： To study the effects of heat shock protein 70 （ HSP70 ） induced by the heat stress pretreatment on inflammation after hepatic ischemia/resperfusion. METHODS： With the hepatic local ischemia/reperfusion model （IR group）, heat stress pretreatment （ H ＋ IR group） and injecting quercetin before heat stress pretreatment （ Q ＋ H ＋ IR group） were performed. The HSP70, intercellular adhesion molecule - 1 （ICAM - 1 ） and the myeloperoxidase （MPO） activity were detected. The levels of serum ALT and AST and histological changes of the hepatocytes were also observed. RESULTS： In H ＋ IR group, the HSP70 expression was higher than that in other groups at each time point, after performing ischemia - perfusion, hepatic injury was slighter. The levels of serum ALT and AST were increased slightly （ P 〈 0. 01 ）. The expression of ICAM - 1 and the changes of MPO activity increased and peaked respectively at 6 h and 12 h after reperfusion. However, they were lower in H ＋ IR group than those in IR group and Q ＋ H ＋ IR group. CONCLUSION： The HSP70 induced by heat stress pretreament reduces the expression of ICAM - 1 and the changes of MPO activity during hepatic ischemia - reperfusion, then hepatic injury is depressed from the inflammation.