左旋黄皮酰胺在大鼠体内的排泄

Excretion of(-)-clausenamide in rats

Aim To study the excretion of(-)-clausenamide in rats.Methods The urine,feces and bile were collected at predetermined time points after(-)-clausenamide was orally administrated to 6 rats(30 mg·kg^-1).The concentrations of(-)-clausenamide and its metabolite 6-OH-(-)-clausnamide were determined by HPLC-MS/MS method using glipzide as the internal reference,and the accumulative excretion amount of(-)-clausenamide and 6-OH-(-)-clausenamide was calculated in the urine,feces and bile,separately.Results(-)-Clausenamide was recovered mostly(44%) from feces in 112 hours,7.1% was found from urine in 120 hours and 0.013% was detected from bile in 24 hours.The accumulative excretions of 6-OH-(-)-clausenamide were 0.92%,0.46% and 0.0003% of the administered dose from feces,urine and bile,respectively.Conclusion The major amount of(-)-clausenamide was recovered from feces after(-)-clausenamide was orally administrated to rats(30 mg·kg^-1).

Aim To study the excretion of （ - ）-clausenamide in rats. Methods The urine, feces and bile were collected at predetermined time points after （ - ）-clausenamide was orally administrated to 6 rats （30 mg·kg^-1 ）. The concentrations of （ - ）-clausenamide and its metabolite 6-OH-（ - ）- clausnamide were determined by HPLC-MS/MS method using glipzide as the internal reference, and the accumulative excretion amount of （ - ）-clausenamide and 6-OH-（ - ）-clausenamide was calculated in the urine, feces and bile, separately. Results （ - ）-Clausenamide was recovered mostly （44%） from feces in ll2 hours, 7. 1% was found from urine in 120 hours and 0. 013% was detected from bile in 24 hours. The accumulative excretions of 6-OH-（ - ）-clausenamide were 0.92% , 0.46% and 0.0003% of the administered dose from feces, urine and bile, respectively. Conclusion The major amount of （ - ）- clausenamide was recovered from feces after （ - ）-clausenamide was orally administrated to rats （30 mg·kg^-1 ）.