包头地区过氧化物酶体增殖体受体γ2基因多态性与2型糖尿病并发冠心病的相关性研究

Relationship between the polymorphism of PPAR-γ2 gene and coronary heart disease in type 2 diabetes mellitus in Baotou

目的：探讨包头地区过氧化物酶体增殖体受体γ2（PPAR—γ2）Pro12Ala基因多态性与2型糖尿病（2DM）并发冠心病（CHD）的相关性。方法：入选对象242例，其中2DM患者48例，2DM并发CHD患者49例，CHD患者45例，另设正常对照组100例。应用聚合酶链反应-限制性片段长度多态性分析进行基因型测定。结果：2DM并发CHD患者基因变异频率明显高于正常对照组（χ^2=4．08，P〈0．05）。PPAR—γ2Pro12Ala等位基因携带者（X／A型）是2DM并发CHD的独立危险因素，OR=2．457，95％CI=1．596～3．782。结论：包头地区PPAR—γ2Pro12Ala等位基因可以增加一般人群2DM并发CHD的患病风险。

Objective：To investigate the relationship between the PPAR-72 Pro12Ala polymorphism and coronary heart disease（CHD） in type 2 diabetes mellitus（2 DM） in Baotou area. Method.Two hundred and forty-two subjects were selected, including 48 patients with type 2 DM,49 type 2 DM patients with CHD,45 patients with CHD and 100 healthy subjects as control. The Prol2Ala polymorphism was screened by means of polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） in all subjects. Result： （1）The frequency of the Pro12Ala mutation was significantly higher in subjects of CHD in type 2 DM than in subjects of control （0. 2857 vs 0. 14）. （2）The genotype carrying Ala allele were indepent risk factor in CHD in type 2 DM （ OR = 2. 457, 95% CI =1. 596-3. 782）. Conclusion：The Ala allele is associated with the increased risk for the development of CHD in type 2 DM in Baotou area.