Repairing articular cartilage defects with tissue-engineering cartilage in rabbits

客观：为了调查 cancellous 的效果，骨头矩阵明胶(BMG ) 在在 rabbits.Methods 修理关节的软骨缺点与 allogeneic 设计了 chondrocytes：Chondrocytes 被播种到三维的 cancellous BMG 上并且在为 12days 到的 vitro 有教养准备 BMG-chondrocyte 建筑群。在有 2.5% pentobarbital 钠(1ml/kg 身体重量) 的麻醉下面，关节的软骨缺点在 38 只健康 NewZealand 白兔子的右膝关节上被做(不管性别，年老的 4-5 月和称的 2.5-3 kg ) 并且缺点然后与 2.5% 胰岛素被对待。然后 BMG-chondrocyte 建筑群(组 A， n = 18 ) ， BMG (组 B， n = 10 ) ，并且没有东西(组 C， n = 10 ) 分别地被植入进软骨缺点。Therepairing 效果被估计由宏观， histologic，传播电子显微镜(TEM ) 观察，免疫组织化学的检查并且在 situ 杂交察觉，分别地在在操作以后的 2， 4， 8， 12 和 24 个星期。结果：Cancellous BMG 在操作以后在 8 个星期以内被降级。在组 A，淋巴细胞渗入在接枝附近被观察。在在操作以后的 24 个星期，软骨缺点被软骨纸巾和关节的软骨和代用品修理软骨的骨头很熟被愈合。Proteoglycan 和类型Ⅱ骨胶原被 Safranin-O 染色和免疫分别地的组织化学的染色在修理纸巾的矩阵检测。在 situ，杂交在修理纸巾在细胞质 ofchondrocytes 证明了类型Ⅱ骨胶原的基因表示。TEM 观察证明在修理纸巾的那个 chondrocytes 和软骨矩阵作为在正常关节的软骨的那些几乎一样。在组 B，缺点被软骨含纤维的纸巾修理。在组 C，缺点仅仅由含纤维的纸巾被修理。结论：Cancellous BMG 能为软骨织物工程被认为是自然房间脚手架。关节的软骨缺点能被 cancellous BMGengineered 与 allogeneic chondrocytes 修理。修理纸巾的性质接近正常软骨。胰岛素的本地管理能支持修理纸巾的坚持招待纸巾。allogeneic chondrocytes 的移植有 immunogenicity，但是有免疫力的反应是弱的。

Objective ： To investigate the effect of cancellous bone matrix gelatin （ BMG ） engineered with allogeneic chondrocytes in repairing articular cartilage defects in rabbits. Methods： Chondrocytes were seeded onto threedimensional cancellous BMG and .cultured in vitro for 12 days to prepare BMG-chondrocyte complexes. Under anesthesia with 2.5% pentobarbital sodium （ lml/kg body weight）, articular cartilage defects were made on the right knee joints of 38 healthy New Zealand white rabbits （regardless of sex, aged 4-5 months and weighing 2. 5-3 kg） and the defects were then treated with 2.5% trypsin. Then BMG-chondrocyte complex （Group A, n = 18）, BMG （Group B, n =10）, and nothing （Group C, n =10） were implanted into the cartilage defects, respectively. The repairing effects were assessed by macroscopic, histologic, transmission electron microscopic （TEM） observation, immunohistochemical examination and in situ hybridization detection, respectively, at 2, 4, 8, 12 and 24 weeks after operation. Results.. Cancellous BMG was degraded within 8 weeks after operation. In Group A, lymphocyte infiltration was observed around the graft. At 24 weeks after operation, the cartilage defects were repaired by cartilagetissues and the articular cartilage and subchondral bone were soundly healed. Proteoglycan and type II collagen were detected in the matrix of the repaired tissues by Safranin-O staining and immunohistochemical staining, respectively. In situ hybridization proved gene expression of type II collagen in the cytoplasm of chondrocytes in the repaired tissues, TEM observation showed that chondrocytes and cartilage matrix in repaired tissues were almost same as those in the normal articular cartilage. In Group B, the defects were repaired by cartilage-fibrous tissues. In Group C, the defects were repaired only by fibrous tissues. Conclusions ： Cancellous BMG can be regarded as the natural cell scaffolds for cartilage tissue engineering. Articular cartilage defects can be repaired by cancellous BMG engineered with allogeneic chondrocytes. The nature of repaired tissues is closest to the normal cartilage. Local administration of trypsin can promote the adherence of repaired tissues to host tissues. Transplantation of allogeneic chondrocytes has immunogenicity, but the immune reaction is weak.