牙胚组织原位杂交方法学探讨

In situ hybridization methodology study with teeth germs

目的探讨牙胚组织原位杂交的最佳方法。方法用牙本质涎磷蛋白DSPP特异性寡核苷酸探针,检测不同时期牙胚DSPPmRNA的表达。结果各种牙胚的成牙本质细胞和前成釉细胞均检测到DSPPmRNA的表达,各阴性对照组均未检测到信号。结论所用的牙胚组织原位杂交方法敏感性高,特异性高。

Objeodve To discuss the best method for in situ hybridization with teeth germs. Methods Oligonucleotide probes labeled with digoxigenin were used in in situ hybridization to inspect DSPP mRNA expression in various teeth germs, Results DSPP mRNA were detected in odontoblasts and preameloblasts in various tooth germs. There was no positive result detected in all negative control groups. Conclusion In situ hybridization method established with teeth germs showed sensitivity and specificity.