肥胖儿童血清脂蛋白（a）水平及载脂蛋白（a）的遗传表型研究

Studies on Sera Lipoprotein(a) Levels and Genetic Phenotypes of Apolipoprotein(a) in Obese Children

本文对肥胖儿血清脂蛋白（ａ）水平及血清载脂蛋白（ａ）多态性进行分析，以探讨肥胖儿与高脂蛋白（ａ）血症的关系及其血清载脂蛋白（ａ）遗传表型的分布特点。用酶联免疫吸附法测定５７名肥胖儿及其双亲血清脂蛋白（ａ）的含量，用十二烷基磺酸钠一聚丙烯酰胺凝胶电泳和Ｗｅｓｔｅｒｎｂｌｏｔ免疫印迹技术测定载脂蛋自（ａ）遗传表型。结果发现高脂蛋白（ａ）血症的肥胖儿中约有７５％的患儿其父母中有一方伴发高脂蛋白（ａ）血症；在４１名肥胖儿中共检出７种载脂蛋白（ａ）遗传表型（其中５种单异构体表型，２种双异构体表型）．肥胖儿中以低分子量的Ｓ１和Ｓ２表型为主；非肥胖儿以高分子量的Ｓ４在型为主，在近５年文献一尚未见类似报道。

This report presents the studies ofsera levels of lipoprotein(a) [Lp (a)] and genetic polymorphism of apolipoprotein(a)[Fapo(a)] in obese children. The aims of this study were to explore the relationship between obese children and high sera levelsof Lp(a) and the distributive characteristics of geneticphenotypes of apo(a).Enzyme-linked immunosorbent assay(ELISA) was used to measure sera levels of Lp(a)in 57 obese children and their parents. The genetic phenotypes of apo (a) were determined by SDS-polyacrylamide gel electrophoresis(SDS-PAGE)and Western blot analysis, Of 75%subjects of obese children with high Lp(a) level, one side of their parents had high concentration of sera Lp(a).A total of 7 different phenotypes of apo(a) were determined from 41 obese children , including 5 different phenotypes of single-isoform and 2 different phenotypes of two-isoform,Obese children mostly had S1 and S2 phenotypes,but the controls generally had S4 phenotype.The similar reports have not been found in the recent5-year references. The study of genetichenotypes of apo(a) in obese children can be of great importance to protection and treatment of the cardiovascular and cerebrovascular disease induced by obesity.