摘要
背景与目的抗肿瘤药物主要通过诱导细胞凋亡发挥作用,化疗耐药性产生可能与凋亡相关基因表达异常有关。本文探讨凋亡抑制基因survivin在胃癌化疗耐药中的作用。方法21.96mmol/L羟基喜树碱处理胃癌细胞株BGC-823,FCM及TUNEL检测细胞凋亡,RT-PCR和免疫组化检测survivin表达。结果与对照组相比,羟基喜树碱能诱导胃癌细胞株BGC-823凋亡。[凋亡率FCM(12.68±0.17)%vs(3.35±0.13)%(P<0.01),凋亡指数(AI)TUNEL法(16.00±1.23)%vs(2.78±0.84)%(P<0.01)],同时诱导BGC-823细胞Survivin表达上调[mRNA(1.489±0.041)vs(0.756±0.037)(P<0.05),蛋白(0.928±0.046)vs(0.303±0.032)(P<0.01)]。结论Survivin基因可能和胃癌细胞获得性化疗耐药有关。
Background and purpose： The antitumor drugs produce a marked effect through the induction of apoptosis. The resistance to chemotherapy has been found to be associated with abnormal expression of genes related to apoptosis. We designed this experiment to investigate the role of survivin gene in the resistance to chemotherapy. Methods： After being treated with Hydroxycamptothecine （OPT, 21.96mmol/L）, the apoptosis in gastric cancer cell line BGC-823 cells were evaluated by either flow cytometry（FCM） or the terminal deoxynucleotidy transfer mediated dUTP-biotin nick end labeling （ TUNEL）. The expression of Survivin was detected by reverse transcription- Polymerase chain reaction（RT-PCR） and immunohistochemistry. Results： Compared to control group, the OPT can induced apoptosis in BGC-823[ （ 12.68 ±0.17） % vs （ 3.35 ± 0.13） % by FCM, （ 16.00 ± 1.23） % vs （2.78 ± 0.84） % by TUNEL[, OPT could upregulate survivin expression in BGC-823 cells （ 1. 489 ±0. 041） % vs （0. 756±0. 037） % in terms of level of mRNA, （0. 928 ±0. 046） vs （0.303±0. 032） in terms of protein expression）. Conclusions： The survivin gene may be associated to the resistance to chemotherapy through the induction of gene expression.
出处
《中国癌症杂志》
CAS
CSCD
2006年第9期741-743,共3页
China Oncology
