蝎毒联合ADM对人乳腺癌MCF-7细/胞的凋亡诱导作用及其耐药逆转的研究

Effect of Inducing Apoptosis and Resistance Reversing of BMK Combined with ADM on Human Breast Cancer MCF-7/ADM Cell Line

目的探讨蝎毒(BMK)联合阿霉素(ADM)对人乳腺癌MCF-7/ADM细胞的凋亡诱导及其耐药逆转作用。方法MTT法、荧光分光光度法、流式细胞术、紫外分光光度术和免疫细胞化学法。结果①非细胞毒性剂量(3.0μg/m l)蝎毒能显著降低MCF-7/ADM的IC50(P<0.01),增加MCF-7/ADM细胞内ADM的药物积累(P<0.01)。②蝎毒(3.0μg/m l)联合ADM后增强了对耐药细胞的凋亡诱导作用,凋亡率由8.9±0.01%上升为12.6±0.21%(P<0.01)。③蝎毒(3.0μg/m l)能够显著降低耐药细胞内谷胱甘肽S转移酶(GSTs)酶的活性(P<0.01)。结论蝎毒能够部分逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性,其逆转机制与抑制耐药细胞GSTs活性有关。

Objective To study the effect of inducing apoposis and resistance reversing of buthus martensii karsch（BMK） combined with adriamycin （ADM） on human breast cancer MCF-7/ADM cell line. Methods The MTT assay, fluorescent-spectrophotometry, flow cytometry, UV-spectropbotometry and immunocytochemistry were used. Results ①The non-cytotoxic dose of BMK （μg/ml） significantly decreased the IC50 value of ADM and increased the intracellular ADM accumulation in MCF-7/ADM cells （ P 〈 0.01, P 〈 0.05 ）. ②BMK（3μg/ml） combined with ADM increased the apoptosis inducing effect of ADM to MCF-7/ADM cells, which of apoptosis percentage was increased from （8.9 ± 0.01 ） % to （ 12.6 ± 0.21 ） % （ P 〈 0.01 ）. ③BMK （3μg/ml） significantly decreased glutathione-S-transferase （GFTs） activity in MCF-7/ADM cells（ P 〈0.01 ）. Conclusion BMK could partially reverse resistance to ADM in human breast cancer MCF-7/ADM cell line, which may be associated with the decreased GSTs activity.