低氧环境下罗勒多糖对乳腺癌细胞TIMPs mRNA表达的影响

Effect of basil polysaccharide on mRNA expression of tissue inhibitor of metalloproteinases in human breast cancer cell line cultured under hypoxia

目的:探讨低氧对人乳腺癌细胞株MDA-231金属蛋白酶组织抑制剂(TIMPs)mRNA表达的影响,以及罗勒多糖(basilpolysaccharide,BP)对TIMPs的作用。方法:分别置MDA-231细胞株于常氧(21%O2、5%CO2)、低氧(1%O2、5%CO2和94%N2)环境中和罗勒多糖(200μg/ml)培养6h,RT-PCR技术检测不同处理组细胞TIMP1、2、3mRNA水平。结果:MDA-231细胞株表达TIMP1、2mRNA,未检测到TIMP3mRNA;低氧环境下MDA-231细胞株TIMP1、2mRNA水平显著上升(P<0.05);罗勒多糖处理后TIMPsmRNA的含量,无论常氧组还是低氧组都有显著改变:常氧环境下罗勒多糖可明显下调MDA-231细胞株中TIMP1、2mRNA的表达(P<0.05),而低氧环境下则显著上调其表达(P<0.05)。结论:罗勒多糖在常氧及低氧环境下对MDA-231细胞TIMP1、2基因表达的影响效应截然相反,提示抗肿瘤药物及生物疗法的体外机制研究设计应充分考虑氧环境的影响。

Objective： To investigate the effect of hypoxia on the mRNA expression of tissue inhibitor of metalloproteinases （TIMPs） in breast carcinoma cell line （MDA-231） and the effect of basil polysaccharide （BP） on the expression of TIMPs. Methods： MDA-231 cells were cultured under normoxia（21% O2, 5% CO2）, hypoxia（1% O2, 5% CO2 and 94% N2 ） or were treated with BP for 6 h separately. The expression of TIMP1, 2, 3 mRNA was detected by RT-PCR. Results： Expression of TIMP1, 2 mRNA, but not TIMP3 mRNA, was detected in MDA-231 cells. After cultured in hypoxia condition for 6 h, the expression of TIMP1 and TIMP2 mRNA increased significantly （ P〈0.05）. The expression of TIMPs mRNA changed significantly in both hypoxia and normoxia group after BP treatment： the expression of TIMP1, 2 mRNA in normoxia group decreased significantly （ P〈0.05 ） and that in hypoxia increased significantly （ P〈0.05 ）. Conclusion： BP has opposite influence on expression of TIMP1, 2 in human breast cancer cell line MDA-231 under hypoxia and normoxia conditions, indicating that in vitro study on anti-tumor drugs and bio-therapy should take the oxygen environment into consideration.